Cytotoxic potential of lung CD8(+) T cells increases with chronic obstructive pulmonary disease severity and with in vitro stimulation by IL-18 or IL-15

TitleCytotoxic potential of lung CD8(+) T cells increases with chronic obstructive pulmonary disease severity and with in vitro stimulation by IL-18 or IL-15
Publication TypeJournal Article
Year of Publication2010
AuthorsFreeman CM, Han MLK, Martinez FJ, Murray S, Liu LX, Chensue SW, Polak TJ, Sonstein J, Todt JC, Ames TM, Arenberg DA, Meldrum CA, Getty C, McCloskey L, Curtis JL
JournalJ Immunol
Volume184
Issue11
Pagination6504-13
Date Published2010 Jun 1
ISSN1550-6606
KeywordsAged, Antigens, CD, Antigens, Differentiation, T-Lymphocyte, CD8-Positive T-Lymphocytes, Cell Separation, Cytokines, Cytotoxicity, Immunologic, Female, Flow Cytometry, Forced Expiratory Volume, Humans, Interleukin-15, Interleukin-18, Lectins, C-Type, Male, Middle Aged, Pulmonary Disease, Chronic Obstructive, Respiratory Function Tests, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger, T-Lymphocyte Subsets
Abstract

Lung CD8(+) T cells might contribute to progression of chronic obstructive pulmonary disease (COPD) indirectly via IFN-gamma production or directly via cytolysis, but evidence for either mechanism is largely circumstantial. To gain insights into these potential mechanisms, we analyzed clinically indicated lung resections from three human cohorts, correlating findings with spirometrically defined disease severity. Expression by lung CD8(+) T cells of IL-18R and CD69 correlated with severity, as did mRNA transcripts for perforin and granzyme B, but not Fas ligand. These correlations persisted after correction for age, smoking history, presence of lung cancer, recent respiratory infection, or inhaled corticosteroid use. Analysis of transcripts for killer cell lectin-like receptor G1, IL-7R, and CD57 implied that lung CD8(+) T cells in COPD do not belong to the terminally differentiated effector populations associated with chronic infections or extreme age. In vitro stimulation of lung CD8(+) T cells with IL-18 plus IL-12 markedly increased production of IFN-gamma and TNF-alpha, whereas IL-15 stimulation induced increased intracellular perforin expression. Both IL-15 and IL-18 protein expression could be measured in whole lung tissue homogenates, but neither correlated in concentration with spirometric severity. Although lung CD8(+) T cell expression of mRNA for both T-box transcription factor expressed in T cells and GATA-binding protein 3 (but not retinoic acid receptor-related orphan receptor gamma or alpha) increased with spirometric severity, stimulation of lung CD8(+) T cells via CD3epsilon-induced secretion of IFN-gamma, TNF-alpha, and GM-CSF, but not IL-5, IL-13, and IL-17A. These findings suggest that the production of proinflammatory cytokines and cytotoxic molecules by lung-resident CD8(+) T cells contributes to COPD pathogenesis.

DOI10.4049/jimmunol.1000006
Alternate JournalJ. Immunol.
PubMed ID20427767
PubMed Central IDPMC4098931
Grant ListK24 HL004212 / HL / NHLBI NIH HHS / United States
K24 HL04212 / HL / NHLBI NIH HHS / United States
KL2 RR024987 / RR / NCRR NIH HHS / United States
N01 HR046162 / HR / NHLBI NIH HHS / United States
N01 HR046162 / HR / NHLBI NIH HHS / United States
P30 CA46952 / CA / NCI NIH HHS / United States
R01 HL082480 / HL / NHLBI NIH HHS / United States
R01 HL082480 / HL / NHLBI NIH HHS / United States
T32 HL07749 / HL / NHLBI NIH HHS / United States