Novosphingobium and its potential role in chronic obstructive pulmonary diseases: insights from microbiome studies

TitleNovosphingobium and its potential role in chronic obstructive pulmonary diseases: insights from microbiome studies
Publication TypeJournal Article
Year of Publication2014
AuthorsRutebemberwa A, Stevens MJ, Perez MJ, Smith LP, Sanders L, Cosgrove G, Robertson CE, Tuder RM, J Harris K
JournalPLoS One
Volume9
Issue10
Paginatione111150
Date Published2014
ISSN1932-6203
KeywordsAdult, Aged, Alphaproteobacteria, Animals, Bronchoalveolar Lavage, Cell Separation, Female, Flow Cytometry, Humans, Inflammation, Lung, Macrophages, Male, Mice, Mice, Inbred C57BL, Microbiota, Middle Aged, Neutrophils, Polymerase Chain Reaction, Pulmonary Disease, Chronic Obstructive, Sequence Analysis, DNA, Smoke, Smoking
Abstract

Bacterial infection of lung airways underlies some of the main complications of COPD, significantly impacting disease progression and outcome. Colonization by bacteria may further synergize, amplify, or trigger pathways of tissue damage started by cigarette smoke, contributing to the characteristic airway inflammation and alveolar destruction of COPD. We sought to elucidate the presence and types of lung bacterial populations in different stages of COPD, aimed at revealing important insights into the pathobiology of the disease. Sequencing of the bacterial small subunit ribosomal RNA gene in 55 well-characterized clinical lung samples, revealed the presence of Novosphingobium spp. (>2% abundance) in lungs of patients with GOLD 3-GOLD 4 COPD, cystic fibrosis and a subset of control individuals. Novosphingobium-specific quantitative PCR was concordant with the sequence data and high levels of Novosphingobium spp. were quantifiable in advanced COPD, but not from other disease stages. Using a mouse model of subacute lung injury due to inhalation of cigarette smoke, bronchoalveolar lavage neutrophil and macrophage counts were significantly higher in mice challenged intratracheally with N. panipatense compared to control mice (p<0.01). Frequencies of neutrophils and macrophages in lung tissue were increased in mice challenged with N. panipatense at room air compared to controls. However, we did not observe an interaction between N. panipatense and subacute cigarette smoke exposure in the mouse. In conclusion, Novosphingobium spp. are present in more severe COPD disease, and increase inflammation in a mouse model of smoke exposure.

DOI10.1371/journal.pone.0111150
Alternate JournalPLoS ONE
PubMed ID25340840
PubMed Central IDPMC4207766
Grant ListR01 ES016285 / ES / NIEHS NIH HHS / United States
R01 ES016285 / ES / NIEHS NIH HHS / United States
R03 HL 097009 / HL / NHLBI NIH HHS / United States
R21 HL110721 / HL / NHLBI NIH HHS / United States