Qualitative and quantitative analysis of retinol, retinyl esters, tocopherols and selected carotenoids out of various internal organs form different species by HPLC.

TitleQualitative and quantitative analysis of retinol, retinyl esters, tocopherols and selected carotenoids out of various internal organs form different species by HPLC.
Publication TypeJournal Article
Year of Publication2010
AuthorsSchäffer MW, Sinha RS, Mukherjee S, Nohr D, Wolter M, Biesalski HK, Ong DE, Das SK
JournalAnal Methods
Volume2
Pagination1320-1332
Date Published2010
ISSN1759-9660
Abstract

We report the validation of a reversed-phase gradient HPLC method allowing simultaneous quantification of retinol, retinyl esters, tocopherols and selected carotenoids in lung, liver and plasma of mouse, rat and guinea pig (gp) using a diode array detector. A significant species difference was observed regarding the distribution of retinol and retinyl esters. The levels of total retinol in lung, liver and plasma were in the following order: mouse > rat > gp; rat >mouse > gp; and gp > rat > mouse, respectively. Furthermore, comparison studies revealed similarities between the vitamin A profiles of human and gp lung samples.

DOI10.1039/c0ay00288g
Alternate JournalAnal Methods
PubMed ID20976035
PubMed Central IDPMC2957904
Grant ListR03 HL095419 / HL / NHLBI NIH HHS / United States
R03 HL095419-01A1 / HL / NHLBI NIH HHS / United States
R03 HL095419-02 / HL / NHLBI NIH HHS / United States