Requests

Thank you for your interest in receiving resources from the Lung Tissue Research Consortium (LTRC).  The LTRC can provide a variety of lung specimens that may be diagnostic of fibrotic and non-fibrotic lung disease, resulting from lung biopsies, lobectomies, transplant procedures and Lung Volume Reduction Surgeries (LVRS).

The LTRC obtains surgical waste tissues from individuals undergoing lung resection or biopsy for clinical indications. The average age of individuals undergoing surgical resection in the LTRC repository is approximately 60 years. As such, the LTRC does not provide access to completely normal lung tissues. The investigator should be aware of this when requesting tissues from the LTRC repository. Appropriate controls for most studies of COPD would consist of peripheral lung tissues resected at time of nodule resection from patients with normal lung function testing parameters. However, such tissues may have some microscopic evidence of minimal centrilobular emphysema or respiratory bronchiolitis. These findings would be reviewed and scored as minimal by the LTRC lung pathologist. Appropriate controls for interstitial lung disease could also include similar tissues from patients undergoing nodule resection. Alternatively, investigators might evaluate tissue changes across patients with increasing severity of physiologic impairment related to their underlying disease (either COPD or IPF), contrasting changes in those with mild disease to those with more severe impairment.

Additional information regarding LTRC specimen requests is provided below. Click the links to download the Request Application relevant to your investigative needs. Information regarding specimen collection protocols can be found on the Protocols page. A summary table of LTRC request applications which have been approved is available here.

Specimens may be requested by completing the Standard Materials Request application. 

Small amounts (10 or less) of specimens for pilot studies may be requested by completing the Express Request application.

Access to just phenotypical data may be requested by completing the Data Request application. Note if specimens are also being requested and you wish to gain access to phenotypical data related to those specimens, a separate Data Request application is not needed. You may indicate the requested data in your Standard Materials/Express Request application.

Request Application Submission:

To submit your request, follow the steps below. If you are a new investigator, begin at step 1. If you are a returning investigator, begin at step 3.

1. You will first need to request an account to access the secure investigator area of the website. To do so, click “Contact Us” located in the upper right-hand corner of the page.

2. Enter “Request New Account” in the body field and complete the other required information. You will be emailed instructions on how to access your account typically within 24 hours. Continue following the next steps below.

3. Log into the LTRC website with your Username and Password.

4. Click on “Request Review” then “New Application” and follow the directions to upload your request application.

5. After your request is broadcast to the consortium you can track its status in the review process by clicking on “Request Review” from the menu. A link to your request will appear under the “My Requests” table and you provide supplemental materials as needed using the “Add” button next to your request.

Review Process:

All requests undergo a review process in the order they are received in. The Data Coordinating Center first processes the application to ensure clarity and completeness prior to broadcasting to the LTRC Protocol Review Committee and NHLBI. Please note that following the broadcast, the committee requires at least two weeks to complete their review of each specimen and/or letter of support request. Upon approval, the letter or specimens will be shipped to your laboratory as such specimens can be prepared by the LTRC Tissue Core Laboratory.

Horizontal Tabs

Tissue Blocks
Specimen Types Tissue samples available Approximate number of samples per patient from tissue harvested Standard aliquot per patient for distribution Uses
Formalin Fixed, Paraffin-Embedded Tissue blocks 1/biopsy
4-10/lobe
12-30/explant
1 block Histopathology, DNA
Slides
4-5 microns
100/block ≤ 20 slides Histopathology, DNA
HOPE Fixed Tissue blocks 1/biopsy
1-3/lobe
6-15/explant
1 block Histopathology, DNA, RNA, protein
slides 100/block ≤ 20 slides Histopathology, DNA
"slices"
20 microns
25/block ≤ 5 slices DNA, RNA, protein
Aliquots
Specimen Types Tissue samples available Approximate number of samples per patient from tissue harvested Standard aliquot per patient for distribution Uses
RNA Later
RNA Preserved
Tissues
aliquots 10/lobe
10-25/explant
30-100 mg DNA, RNA, protein
Flash frozen aliquots 5/lobe
10-25/explant
30-100 mg
or
100-1000 mg
DNA, RNA, protein
Glutaraldehyde aliquots 1-3/lobe 1 mm³ electron
microscopy
Blood
Specimen Types Tissue samples available Approximate number of samples per patient from tissue harvested Standard aliquot per patient for distribution
Plasma/Serum aliquots 4 ml 0.5 ml
DNA aliquots 150-500 mcg 20 mcg