Requests

IMPORTANT: The LTRC Data Coordinating Center contract with NHLBI has ended effective September 1, 2020. Applications for access to LTRC data and specimens are not being accepted at this time. NHLBI will make LTRC specimens and data available via its BioLINCC repository in the coming months. Information on how to request LTRC resources through BioLINCC is forthcoming. Please check NHLBI's website https://www.nhlbi.nih.gov/science/lung-tissue-research-consortium-ltrc for updates.

Horizontal Tabs

Tissue Blocks
Specimen Types Tissue samples available Approximate number of samples per patient from tissue harvested Standard aliquot per patient for distribution Uses
Formalin Fixed, Paraffin-Embedded Tissue blocks 1/biopsy
4-10/lobe
12-30/explant
1 block Histopathology, DNA
Slides
4-5 microns
100/block ≤ 20 slides Histopathology, DNA
HOPE Fixed Tissue blocks 1/biopsy
1-3/lobe
6-15/explant
1 block Histopathology, DNA, RNA, protein
slides 100/block ≤ 20 slides Histopathology, DNA
"slices"
20 microns
25/block ≤ 5 slices DNA, RNA, protein
Aliquots
Specimen Types Tissue samples available Approximate number of samples per patient from tissue harvested Standard aliquot per patient for distribution Uses
RNA Later
RNA Preserved
Tissues
aliquots 10/lobe
10-25/explant
30-100 mg DNA, RNA, protein
Flash frozen aliquots 5/lobe
10-25/explant
30-100 mg
or
100-1000 mg
DNA, RNA, protein
Glutaraldehyde aliquots 1-3/lobe 1 mm³ electron
microscopy
Blood
Specimen Types Tissue samples available Approximate number of samples per patient from tissue harvested Standard aliquot per patient for distribution
Plasma/Serum aliquots 4 ml 0.5 ml
DNA aliquots 150-500 mcg 20 mcg