All fixed and frozen tissues that are obtained as part of
the LTRC study are prepared according to the instructions given in the LTRC Manual
of Procedures (MOP).
Ideally, 100 mg of tissue is collected per fixative. If there is not sufficient
tissue for at least one 100 mg aliquot per fixative, sites will priotize a
frozen preparation, followed by RNALater, followed by formalin. Note
glutaraldehyde specimens and HOPE fixed specimens were discontinued for the LTRC
II and LTRC III studies.
The LTRC investigators have set as a goal that the ischemic period for LTRC
tissue will be no longer than 15 minutes for Video Assisted Thoracoscopic Lung
Biopsy (VATS) and other biopsy specimens.
All tissue specimens will be sent to the Tissue Core Laboratory and stored as
- RNAIater tissue is aliquoted and stored at -20°C.
- Flash (snap) frozen tissue is aliquoted and stored at -80°C.